My personal favorite was always the one about "English is just a programming language", but when I read the 20th anniversary edition that one seemed like it had been toned down? I did not go back to find the original one to compare, but the way I remember it it was pretty hardcore about keeping text as text and using tools like for programming (use macros in text to avoid repeating yourself etc).
Overall the 20th anniversary did feel a bit less idealistic? I guess for a "pragmatic" book that makes sense, but I remember the original like it was making stronger arguments for or against things. I really liked the (anti-)IDE chapter, or the parts on the importance of learning how to use a good text editor well, for instance, but now they basically cut that out. Did give me the impression that they were trying to be down with the kids at times.