The median sample size of the studies subjected to replication was n = 5 specimens (https://osf.io/atkd7). Probably because only protocols with an estimated cost less than BRL 5,000 (around USD 1,300 at the time) per replication were included. So it's not surprising that only ~ 60% of the original biomechemical assays' point estimates were in the replicates' 95% prediction interval. The mouse maze anxiety test (~ 10%) seems to be dragging down the average. n = 5 just doesn't give reliable estimates, especially in rodent psychology.
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