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owlninja ◴[] No.42728486[source]
I guess I'll bite - what am I looking at here?
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the__alchemist ◴[] No.42728569[source]
An (agarose?) gel.

There are partial holes in at at one end. You insert a small amount of dyed DNA (etc) containing solution each. Apply an electrical potential across the gel. DNA gradually moves along. Smaller DNA fragments move faster. So, at a given time, you can coarsely measure fragment size of a given sample. Your absolute scale is given by "standards", aka "ladders" that have samples of multiple, known sizes.

The paper authors cheated (allegedly) by copy + pasting images of the gel. This is what was caught, so it implies they may have made up some or all results in this and other papers.

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shpongled ◴[] No.42728684[source]
Close - this is a SDS-PAGE gel, and you run it using proteins. The bands in the first two rows are from a western blot (gel is transferred to a membrane), where you use antibodies against those specific proteins to detect them. The Pon S row is Ponceau S, a dye that non-specifically detects all proteins - so it's used as a loading control, to make sure that the same amount of total protein is loaded in each lane of the gel.
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doctorpangloss ◴[] No.42728830{3}[source]
Is it conceivable that the control was run once because the key result came from the same run? I can see a reviewer asking for it in all three figures, whereas they may drafted it only in one
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1. shpongled ◴[] No.42729906{4}[source]
Based on the images, it is inconceivable that these are from the same run (see the dramatically different levels of TRF-S in each gel. One column/lane = one sample). This isn't something that would be included because of a reviewer - loading controls are required to meaningfully interpret the results (e.g. the data is useless without such a control).